Cord Tissue Processing

What is Mesenchymal Stromal Cell Culture Cord Tissue Processing

Did you know that not all cell banks process and store cord tissue the same way? In fact, most cord blood/stem cell banks do not process and store cord tissue together as a cellular product. Rather, they store the cord tissue as a whole or as minced tissue instead of isolating the mesenchymal stromal cells (MSC) prior to storage.

 

Different Methods of Processing and Storing Cord Tissue

Scientists have discovered that the umbilical cord is one of the most concentrated sources of mesenchymal stem cells (MSC). MSC is used in experimental treatments for osteoarthritis and sports injuries, as well as cell therapies that differentiate into various tissues and organ cells, including cardiac, nerve, cartilage/bone, and muscle cells. There are over 300 ongoing MSC clinical trials (ClinicalTrials.gov) and over 6000 articles published, which further encourages the pursuit of huge opportunities to use MSC in future cell-based therapies.

Currently, there are two methods that cell banks use to process cord tissue:

Full/Segmented Cord Storage — The umbilical cord is first collected at birth, then washed several times at the lab, immersed within an appropriate solution carrying a cryoprotectant agent, and lastly cryopreserved. The cryoprotectant agents are molecules that protect tissues and cells from being damaged during the freezing process. As an alternative procedure, the cord can also be cut into segments through a similar processes. With Full/Segmented Cord Storage, MSCs are not isolated from the cord. Instead, the hope is that they can someday be retrieved from the cord tissue with high cell yield and viability.

Cell Culture (Isolated MSC) storage — For the purpose of cell therapy on a clinical scale, many steps are performed to process MSCs from cord tissue: cell isolation, expansion, and culture. Then, the extracted cells are placed in a cryoprotectant agent and immediately cryopreserved.

 

Advantage of HealthBanks’ MSC Culture Cord Tissue Process Method

HealthBanks strives to use our innovative technology and lab techniques to process and store your MSCs. There are many advantages to our process:

  • Higher Cell Count – Once the tissue has been frozen as a whole or in minced segments, it can be hard to separate quality cells from the tissue. This is especially difficult when the cryoprotectant agent, DMSO, does not penetrate the whole unit or minced segments very well. By using the culture method and extracting cells as a cellular product, we can expect higher cell count results.

 

  • Cell Growth – The extracted cells obtained from the tissue are not enough for therapeutic use. They often require further cell growth to ensure future clinical readiness. During our HealthBanks MSC culture processing, we conduct a cell growth test to ensure that your precious cells can continue to grow and will still be useful when they are needed in the future.

 

  • Bacteria Test – When bacteria is present in the cord tissue, the cells become ineligible for future use. Storing a whole tissue unit or segmented minced pieces makes it extremely difficult to conduct a bacteria test. However, our HealthBanks MSC Culture Method conducts a bacteria test after your cells are isolated from your tissue, before they are eligible for storing. Rest assured, you can have full confidence knowing that your cells will be viable when you need them most.

 

Before making any decision about your future, be sure to do your research. When selecting the right cell bank to store your cord tissue, choose an organization that is experienced , accredited, and trusted. HealthBanks is here to help answer any questions you might have and provide the best solution for your future.

References:

Parent’s Guide to Cord Blood Foundation: Storage of Mesenchymal Stem/Stromal cells in family stem cell banks

Subscribe to Our Newsletter & Special Promotions

Disclaimer: The use of cord blood and immune cells is determined by the treating physician and manufacturer of the final products and is influenced by many factors, including the patient's medical condition, the characteristics of the sample, and whether the immune cells should be further modified or expanded. Cord blood has established uses in transplant medicine; however, its use in regenerative medicine is still being researched. There is no guarantee that potential medical applications being studied in the laboratory or clinical trials will become available. Cord tissue use is still in early research stages, and there is no guarantee that treatments using cord tissue will be available in the future. Cord tissue is stored whole. Additional processing prior to use will be required to extract and prepare any of the multiple cell types from cryopreserved cord tissue. Immune cells has established uses in certain blood cancer treatment; however, its use in solid cancer is still being researched. There is no guarantee that treatments being studied in the laboratory, clinical trials, or other experimental treatments will be available in the future.

HealthBanks cryopreserves immune cells that have been collected from an individual for potential future cellular therapy use. HealthBanks does not provide medical advice, treatment or care. The use of cryopreserved cells for future immunotherapies is not guaranteed. Much is yet unknown about the future of immunotherapy and the immune cells that have been cryopreserved. The use of cryopreserved cells by commercial manufacturers for CAR-T therapy production is not currently approved by the FDA. There is active research ongoing, however, there is no guarantee that your cryopreserved cells will have a future therapeutic use. Only your health care professional can help you evaluate whether your cells might be used and if so, how they might be used. HealthBanks and its shareholders, directors, officers, agents, employees, affiliates, network of providers and provider facilities, will not be liable to you or any other party for claims relating to sample collection or if your cells cannot be used, or if any harm, injury, damage or loss results from the use of your cells. For more information, please refer to HealthBanks Service Agreement.